bio rad geldoc go imaging system  (Bio-Rad)

Journal: medRxiv

Article Title: Circulating eNAMPT in Glaucoma: A Semi-Quantitative Plasma Analysis Before and After Nicotinamide Supplementation

doi: 10.1101/2025.08.15.25333760

Figure Lengend Snippet: A) Schematic overview of the NAD salvage pathway, highlighting various cellular roles, including its function as a mitochondrial electron donor in its reduced form (NADH). B) Schematic representation of the final WB workflow. In summary 1) 10 μL of plasma diluted 1:50 in Pierce Lane Marker Reducing Sample Buffer (1x) were loaded into a 4–20% Criterion TGX Stain-Free 18 well Protein Midi Gel and 2) run for 45 minutes at 200 V in a Criterion Cell. A reference sample is included in 3 positions in each gel for further blot corrections 3) Gel was imaged after electrophoretic separation with Geldoc Go Imaging System for the detection of the Stain-Free technology, allowing the visualization and confirmation of proper protein separation. 4) Gel separated proteins were transferred using the Trans-Blot Turbo Transfer System to a Midi 0.2 µm Nitrocellulose membrane and 5) visualization was again performed to confirm the proper transference and 1 hour blocking was performed at room temperature with slow agitation (∼40-45 rpm). 6) Primary antibody (eNAMPT protein of interest; Transferrin Housekeeping protein) overnight incubation following secondary antibody (IRDye 680RD; IRDye 800CW) 1 hour room temperature incubations were performed in black boxes with slow agitation (∼40-45 rpm). 7) Dynamic range detection of protein fluorescent bands was performed with the Li-Cor CLx imager and quantified with Empiria Studio 3.2 in order to 8) assess the relative eNAMPT expression to transferrin expression. C) Fluorescence images of 2 representative Western Blots. D) Distribution of the normalized relative eNAMPT plasma levels measured in all study cohort samples in each of the 17 performed WB. Normalized relative eNAMPT levels are calculated from the ratio of signals of eNAMPT by transferrin as housekeeping protein and corrected inter-blot wise by the reference sample values. eNAMPT: extracellular nicotinamide phosphoribosyltransferase; NAD: nicotinamide adenine dinucleotide; NAM: nicotinamide; NAMPT: nicotinamide phosphoribosyltransferase; NMN: nicotinamide mononucleotide; NMNATs: nicotinamide-nucleotide adenylyltransferases; NMRKs: nicotinamide riboside kinases NR: nicotinamide riboside; WB: Western blot.

Article Snippet: The blot after transference was imaged with the Geldoc Go Imaging System (Bio-Rad, #12009077) using the Stain-Free Blot mode (1-3 seconds exposure) to confirm the proper protein transfer.

Techniques: Clinical Proteomics, Marker, Staining, Imaging, Membrane, Blocking Assay, Incubation, Expressing, Fluorescence, Western Blot

Journal: STAR Protocols

Article Title: Protocol for the functional evaluation of genetic variants using saturation genome editing

doi: 10.1016/j.xpro.2025.103710

Figure Lengend Snippet:

Article Snippet: Optional: GelDoc Go imaging system , Bio-Rad , Cat#12009077EDU.

Techniques: Virus, Recombinant, Ligation, Staining, Transfection, Modification, Saline, Plasmid Preparation, Purification, Gel Extraction, Variant Assay, Amplification, Sequencing, Sampling, Colony Assay, Software, Real-time Polymerase Chain Reaction, Sterility, Cell Culture, Cell Counting, Electroporation, Imaging